Lachat Method: Ammonia 12-107-06-1-B


Dokument-ID ME148


Veröffentlichungsdatum 18.01.2022
Lachat Method: Ammonia 12-107-06-1-B
Method # Range MDL Units Matrix Rev. Date
12-107-06-1-B 1.0-20.0 0.035 mg/L NH3-N Soil Extracts 15-Sep-08
Exchangeable ammonium is extracted from soil with 2 M KCl. The KCl extract is filtered and the filtrate is analyzed for ammonia by the phenolate method. This method is based on the Berthelot reaction. Ammonia reacts with alkaline phenol, then with sodium hypochlorite to form indophenol blue. Sodium nitroprusside (nitroferricyanide) is added to enhance sensitivity. The absorbance of the reaction product is measured at 630 nm, and is directly proportional to the original ammonia concentration.

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